fgf13a e coli recombinant protein Search Results


fgf13a e coli recombinant protein  (Novus Biologicals)
93
Novus Biologicals fgf13a e coli recombinant protein
LRRC37B is a receptor for <t>FGF13A.</t> A , FGF13 codes for several spliced isoforms; cell lysate and medium samples of HEK-293T cells transfected for FGF13A, FGF13B, FGF13VY and FGF13-core cDNAs (stars indicate in the cell extracts for each isoform). B , LRRC37B-HA immunoprecipitation from HEK-293T cells co-transfected for LRRC37B-HA cDNA and cDNAs coding for the different FGF13 isoforms (stars indicate in the input for each isoform). C , LRRC37B-HA immunoprecipitation from HEK-293T cells transfected for LRRC37B-HA cDNA and with recombinant FGF13A or its synthetic ExonS applied in the culture medium. D-E , Immunoprecipitation of different protein lacking or carrying the leucin-rich repeats (LRR) of the extracellular domain of LRRC37B from transfected HEK-293T cells with recombinant FGF13A application in the culture medium (stars indicate in the input each LRRC37B protein). F, Binding assay of synthetic FGF13A ExonS-biotin to the different LRRC37B proteins carrying or lacking LRR expressed by transfected HEK-293T cells (fitting curves for LRRC37B_ECTO and LRR_ECTO, see Methods). G , Immunoprecipitation of NAV1.6 (SCN8A) from HEK-293T cells transfected for SCN8A/NAV1.6 cDNA with recombinant FGF13A or its synthetic ExonS applied in the culture medium. H , NAV1.6 immunoprecipitations from HEK-293T cells transfected for SCN8A/NAV1.6 +/-FGF13A +/- LRRC37B cDNAs. I , Schematic summary of the LRRC37B – FGF13A – NAV1.6 interaction.
Fgf13a E Coli Recombinant Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fgf13a+e+coli+recombinant+protein/Recombinant+Human+FGF-13+Protein/bio_rxiv__2022__12__21__521423-259-0-7
Average 93 stars, based on 1 article reviews
fgf13a e coli recombinant protein - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


LRRC37B is a receptor for FGF13A. A , FGF13 codes for several spliced isoforms; cell lysate and medium samples of HEK-293T cells transfected for FGF13A, FGF13B, FGF13VY and FGF13-core cDNAs (stars indicate in the cell extracts for each isoform). B , LRRC37B-HA immunoprecipitation from HEK-293T cells co-transfected for LRRC37B-HA cDNA and cDNAs coding for the different FGF13 isoforms (stars indicate in the input for each isoform). C , LRRC37B-HA immunoprecipitation from HEK-293T cells transfected for LRRC37B-HA cDNA and with recombinant FGF13A or its synthetic ExonS applied in the culture medium. D-E , Immunoprecipitation of different protein lacking or carrying the leucin-rich repeats (LRR) of the extracellular domain of LRRC37B from transfected HEK-293T cells with recombinant FGF13A application in the culture medium (stars indicate in the input each LRRC37B protein). F, Binding assay of synthetic FGF13A ExonS-biotin to the different LRRC37B proteins carrying or lacking LRR expressed by transfected HEK-293T cells (fitting curves for LRRC37B_ECTO and LRR_ECTO, see Methods). G , Immunoprecipitation of NAV1.6 (SCN8A) from HEK-293T cells transfected for SCN8A/NAV1.6 cDNA with recombinant FGF13A or its synthetic ExonS applied in the culture medium. H , NAV1.6 immunoprecipitations from HEK-293T cells transfected for SCN8A/NAV1.6 +/-FGF13A +/- LRRC37B cDNAs. I , Schematic summary of the LRRC37B – FGF13A – NAV1.6 interaction.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: LRRC37B is a receptor for FGF13A. A , FGF13 codes for several spliced isoforms; cell lysate and medium samples of HEK-293T cells transfected for FGF13A, FGF13B, FGF13VY and FGF13-core cDNAs (stars indicate in the cell extracts for each isoform). B , LRRC37B-HA immunoprecipitation from HEK-293T cells co-transfected for LRRC37B-HA cDNA and cDNAs coding for the different FGF13 isoforms (stars indicate in the input for each isoform). C , LRRC37B-HA immunoprecipitation from HEK-293T cells transfected for LRRC37B-HA cDNA and with recombinant FGF13A or its synthetic ExonS applied in the culture medium. D-E , Immunoprecipitation of different protein lacking or carrying the leucin-rich repeats (LRR) of the extracellular domain of LRRC37B from transfected HEK-293T cells with recombinant FGF13A application in the culture medium (stars indicate in the input each LRRC37B protein). F, Binding assay of synthetic FGF13A ExonS-biotin to the different LRRC37B proteins carrying or lacking LRR expressed by transfected HEK-293T cells (fitting curves for LRRC37B_ECTO and LRR_ECTO, see Methods). G , Immunoprecipitation of NAV1.6 (SCN8A) from HEK-293T cells transfected for SCN8A/NAV1.6 cDNA with recombinant FGF13A or its synthetic ExonS applied in the culture medium. H , NAV1.6 immunoprecipitations from HEK-293T cells transfected for SCN8A/NAV1.6 +/-FGF13A +/- LRRC37B cDNAs. I , Schematic summary of the LRRC37B – FGF13A – NAV1.6 interaction.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Transfection, Immunoprecipitation, Recombinant, Binding Assay

(related to ). LRRC37 proteins are co-receptors for FGF13A. A, FGF13A co-immunoprecipitates the LRRC37B extracellular part (LRRC37B_ECTO) as well as its leucin-rich repeats (LRR) but not the extracellular part devoid of the LRR from transfected HEK-293T cells. B , Immunoprecipitations of human, chimpanzee, macaque LRRC37B proteins or human LRRC37A2 protein from HEK-293T cells transfected for their cDNA and FGF13A cDNA: all LRRC37 proteins except the macaque LRRC37B binds to FGF13A. C , Immunoprecipitations of LRRC37B_ECTO and other transmembrane proteins (extracellular domain fused at the N-terminal with the prolactin leader peptide and an HA tag, and at the C-terminal with the transmembrane domain of PDGF-R) from HEK-293T cells transfected for their cDNA and FGF13A cDNA; stars indicate the transmembrane protein in the input.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: (related to ). LRRC37 proteins are co-receptors for FGF13A. A, FGF13A co-immunoprecipitates the LRRC37B extracellular part (LRRC37B_ECTO) as well as its leucin-rich repeats (LRR) but not the extracellular part devoid of the LRR from transfected HEK-293T cells. B , Immunoprecipitations of human, chimpanzee, macaque LRRC37B proteins or human LRRC37A2 protein from HEK-293T cells transfected for their cDNA and FGF13A cDNA: all LRRC37 proteins except the macaque LRRC37B binds to FGF13A. C , Immunoprecipitations of LRRC37B_ECTO and other transmembrane proteins (extracellular domain fused at the N-terminal with the prolactin leader peptide and an HA tag, and at the C-terminal with the transmembrane domain of PDGF-R) from HEK-293T cells transfected for their cDNA and FGF13A cDNA; stars indicate the transmembrane protein in the input.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Transfection

LRRC37B interacts with FGF13A in vivo and enhanced its amount at the AIS. A , Immunoprecipitation of LRRC37B-HA from mouse cortical protein extract (P17) of LRRC37B-HA/EGFP transfected mouse cortex compared to EGFP alone. B , STED microscopy of the AIS of mouse neurons transfected for LRRC37B/EGFP or EGFP alone immunostained for EGFP, LRRC37B, FGF13A and NAVα subunits; arrowheads indicate colocalizations between LRRC37B & FGF13A. C, Mouse neurons transfected for LRRC37B/EGFP or EGFP alone immunostained for EGFP and FGF13A. D , Corresponding quantification of FGF13A staining at the soma and AIS levels (Mann-Whitney tests; ns, non-significative; ****, p<0.0001).

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: LRRC37B interacts with FGF13A in vivo and enhanced its amount at the AIS. A , Immunoprecipitation of LRRC37B-HA from mouse cortical protein extract (P17) of LRRC37B-HA/EGFP transfected mouse cortex compared to EGFP alone. B , STED microscopy of the AIS of mouse neurons transfected for LRRC37B/EGFP or EGFP alone immunostained for EGFP, LRRC37B, FGF13A and NAVα subunits; arrowheads indicate colocalizations between LRRC37B & FGF13A. C, Mouse neurons transfected for LRRC37B/EGFP or EGFP alone immunostained for EGFP and FGF13A. D , Corresponding quantification of FGF13A staining at the soma and AIS levels (Mann-Whitney tests; ns, non-significative; ****, p<0.0001).

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: In Vivo, Immunoprecipitation, Transfection, Microscopy, Staining, MANN-WHITNEY

FGF13A regulates neuronal excitability through its ExonS. A , AP firing rate of mouse pyramidal neurons before and after 50nM recombinant FGF13A extracellular application (2-way ANOVA test). B-C , Dose-response effect of recombinant FGF13A extracellular application on cell intrinsic properties (AP firing rate normalized at 700 pA)(paired Wilcoxon tests); normalization for each cells to values before application. D , Phase plot analysis of AP generation before and after 50nM recombinant FGF13A extracellular application (paired Wilcoxon tests). E-F , Intrinsic excitability of mouse pyramidal neurons before and after 50nM synthetic ExonS extracellular application (2-way ANOVA test for AP firing rate; paired Wilcoxon test for rheobase). G , AP properties of mouse pyramidal neurons before and after 50nM synthetic ExonS extracellular application (paired Wilcoxon test). H , Phase plot analysis of AP generation before and after 50nM synthetic ExonS extracellular application (paired Wilcoxon tests). ns, non-significative; *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: FGF13A regulates neuronal excitability through its ExonS. A , AP firing rate of mouse pyramidal neurons before and after 50nM recombinant FGF13A extracellular application (2-way ANOVA test). B-C , Dose-response effect of recombinant FGF13A extracellular application on cell intrinsic properties (AP firing rate normalized at 700 pA)(paired Wilcoxon tests); normalization for each cells to values before application. D , Phase plot analysis of AP generation before and after 50nM recombinant FGF13A extracellular application (paired Wilcoxon tests). E-F , Intrinsic excitability of mouse pyramidal neurons before and after 50nM synthetic ExonS extracellular application (2-way ANOVA test for AP firing rate; paired Wilcoxon test for rheobase). G , AP properties of mouse pyramidal neurons before and after 50nM synthetic ExonS extracellular application (paired Wilcoxon test). H , Phase plot analysis of AP generation before and after 50nM synthetic ExonS extracellular application (paired Wilcoxon tests). ns, non-significative; *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Recombinant

(related to ). FGF13A and its ExonS act extracellularly on neuronal excitability. A-D, Electrophysiological properties complementary to Figure 45A-C, with recombinant FGF13A extracellular application on mouse cortical sections at 0nM, 5nM, 10nM and 50nM (2-way ANOVA tests for AP firing rate, paired Wilcoxon test for others). E , Dose-response (0-50nM) of sodium currents of mouse cortical neurons with recombinant FGF13A extracellular application. F , Electrophysiological properties complementary to , with synthetic ExonS extracellular application on mouse cortical sections at 50nM (2-way ANOVA tests for AP firing rate & sodium currents, paired Wilcoxon tests for others). ns, non-significant; *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: (related to ). FGF13A and its ExonS act extracellularly on neuronal excitability. A-D, Electrophysiological properties complementary to Figure 45A-C, with recombinant FGF13A extracellular application on mouse cortical sections at 0nM, 5nM, 10nM and 50nM (2-way ANOVA tests for AP firing rate, paired Wilcoxon test for others). E , Dose-response (0-50nM) of sodium currents of mouse cortical neurons with recombinant FGF13A extracellular application. F , Electrophysiological properties complementary to , with synthetic ExonS extracellular application on mouse cortical sections at 50nM (2-way ANOVA tests for AP firing rate & sodium currents, paired Wilcoxon tests for others). ns, non-significant; *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Recombinant

(related to ). FGF13A doesn’t act intracellularly on neuronal excitability. A, Cell intrinsic excitability of mouse neurons with 50nM intracellular application of recombinant FGF13A (2-way ANOVA test for AP firing rate, Mann-Whitney test for rheobase). B , AP properties of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann-Whitney tests). C , Electrophysiological properties of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann- Whitney tests) . D, Phase plot analysis of single APs of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann-Whitney tests) . E, Sodium currents of mouse neurons with 50nM intracellular application of recombinant FGF13A (2-way ANOVA). ns, non-significant; *, p<0.05; ****, p<0.0001.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: (related to ). FGF13A doesn’t act intracellularly on neuronal excitability. A, Cell intrinsic excitability of mouse neurons with 50nM intracellular application of recombinant FGF13A (2-way ANOVA test for AP firing rate, Mann-Whitney test for rheobase). B , AP properties of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann-Whitney tests). C , Electrophysiological properties of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann- Whitney tests) . D, Phase plot analysis of single APs of mouse neurons with 50nM intracellular application of recombinant FGF13A (Mann-Whitney tests) . E, Sodium currents of mouse neurons with 50nM intracellular application of recombinant FGF13A (2-way ANOVA). ns, non-significant; *, p<0.05; ****, p<0.0001.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Recombinant, MANN-WHITNEY

LRRC37B binds to SCN1B and prevents NAV1.6/SCN1B interaction. A-B, Immunoprecipitation of different protein lacking or carrying the LB specific domain of the extracellular domain of LRRC37B from HEK-293T cells co-transfected for the SCN1B cDNA. C , SCN1B immunoprecipitations from HEK-293T cells transfected for SCN1B +/- LRRC37B +/- FGF13A cDNAs. D , NAV1.6 immunoprecipitations from HEK- 293T cells transfected for SCN8A/NAV1.6 +/- SCN1B +/- LRRC37B cDNAs: SCN1B binds to NAV.6 which is altered in presence of LRRC37B. E , Schematic summary of the LRRC37B – SCN1B – FGF13A – NAV1.6 interaction.

Journal: bioRxiv

Article Title: LRRC37B is a species-specific regulator of voltage-gated channels and excitability in human cortical neurons

doi: 10.1101/2022.12.21.521423

Figure Lengend Snippet: LRRC37B binds to SCN1B and prevents NAV1.6/SCN1B interaction. A-B, Immunoprecipitation of different protein lacking or carrying the LB specific domain of the extracellular domain of LRRC37B from HEK-293T cells co-transfected for the SCN1B cDNA. C , SCN1B immunoprecipitations from HEK-293T cells transfected for SCN1B +/- LRRC37B +/- FGF13A cDNAs. D , NAV1.6 immunoprecipitations from HEK- 293T cells transfected for SCN8A/NAV1.6 +/- SCN1B +/- LRRC37B cDNAs: SCN1B binds to NAV.6 which is altered in presence of LRRC37B. E , Schematic summary of the LRRC37B – SCN1B – FGF13A – NAV1.6 interaction.

Article Snippet: FGF13A E. coli recombinant protein originates from Novus Biologicals (NBP2-35009).

Techniques: Immunoprecipitation, Transfection